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KMID : 0379120090370020121
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Korean Journal of Mycology
2009 Volume.37 No. 2 p.121 ~ p.129
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Identification of Fusarium Species Associated with Corn Ear Rot
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Choi Hyo-Won
Kim Jung-Mi
Kim Jin-Hee
Hong Sung-Kee
Kim Wan-Gyu
Chun Se-Chul
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Abstract
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In 2007, a total of 77 isolates of Fusarium spp. were obtained from ear rot symptoms of corns collected from 5 locations in Gangwon Province, Korea. The fungal isolates were identified based on their morphological features. Out of the isolates, fifteen isolates were identified as Fusarium verticillioides which formed microconidia in long chains on monophialides. Four isolates were identified as F. subglutinans which formed microconida only on false heads. Six isolates were identified as F. graminearum which produced red pigment in PDA culture. Besides these Fusarium species, F. napiform, F. nygamai, and F. oxysporum were identified from the rest isolates. To assess for genetic diversity of the isolates, a random amplified polymorphic DNA(RAPD) technique was carried out using URP primers. The results from the RAPD analysis showed that the isolates from corn were divided into 6 groups. These RAPD groups of the Fusarium species corresponded to morphological characters of the Fusarium species. The phylogenetic analysis of most isolates by DNA sequencing of EF-1α gene corresponded to morphological characters of the Fusarium species. The results of pathogenicity tests by two inoculation methods revealed that F. verticillioides, F. graminearum and F. subglutinans are strongly pathogenic to corn stalks.
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KEYWORD
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Corn ear rot, Fusarium graminearum, Fusarium subglutinans, Fusarium verticillioides, Pathogenicity
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